Magnetic Microparticle Based Dna Extraction in a Droplet Microfluidic Chip
نویسندگان
چکیده
We are reporting an efficient method for magnetic microparticle based DNA extraction on a segmented flow microfluidic platform [1]. The droplet splitter and the magnetic setup were optimized separately to achieve highest separation efficiency. The novel asymmetric droplet splitter was developed allowing for dynamic control and arbitrary selection of droplet splitting ratios between 50:50 and 95:5. Further, the magnet position and orientation were optimized via numerical modeling and experiments. The optimized microflidic system was used for microparticle-based DNA extraction, resulting in 97% of microbead separation efficiency, 90% DNA capture efficiency, while removing 90% of original volume in single step.
منابع مشابه
A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification.
This paper reports a droplet microfluidic, sample-to-answer platform for the detection of disease biomarkers and infectious pathogens using crude biosamples. The platform exploited the dual functionality of silica superparamagnetic particles (SSP) for solid phase extraction of DNA and magnetic actuation. This enabled the integration of sample preparation and genetic analysis within discrete dro...
متن کاملContinuous microfluidic DNA extraction using phase-transfer magnetophoresis.
This paper reports a novel microfluidic-chip based platform using "phase-transfer magnetophoresis" enabling continuous biomolecule processing. As an example we demonstrate for the first time continuous DNA extraction from cell lysate on a microfluidic chip. After mixing bacterial Escherichia coli culture with superparamagnetic bead suspension, lysis and binding buffers, DNA is released from cel...
متن کاملFluorescent Contrast agent Based on Graphene Quantum Dots Decorated Mesoporous Silica Nanoparticles for Detecting and Sorting Cancer Cells
Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins. Material and Methods: We adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 c...
متن کاملA Microfluidic Chip for Bio-Bar-Code-Based Detection of Proteins
An ultrasensitive nanoparticle-based biomolecule detection method, known as BioBar-Coding (BBC) was recently developed by the Mirkin Group, which utilizes magnetic microparticle probes (MMP) functionalized with monoclonal antibodies that specifically bind to the target of interest [prostate-specific antigen (PSA) in this case; PSA is a prostate cancer marker and a potential breast cancer marker...
متن کاملMicrofluidic synthesis of advanced microparticles for encapsulation and controlled release.
We describe droplet microfluidic strategies used to fabricate advanced microparticles that are useful structures for the encapsulation and release of actives; these strategies can be further developed to produce microparticles for advanced drug delivery applications. Microfluidics enables exquisite control in the fabrication of polymer vesicles and thermosensitive microgels from single and high...
متن کامل